Difference between revisions of "Part:BBa K1154000:Design"

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===Design Notes===
 
===Design Notes===
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This commercially synthesized construct was designed for high codon bias in ''Saccharomyces cerevisiae'' and conformity with current Registry standards. Its component sequences can be found in the Registry (see Source). However, assembling those sequences using the Composite Parts Tool will not produce a construct with sequence identical to that submitted here.
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This construct was designed for high codon bias in ''Saccharomyces cerevisiae'' and conformity with current Registry standards. Its component sequences can be found in the Registry (see Source).
  
 
===Source===
 
===Source===
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This sequence was commercially synthesized. Component sequences can be found in the Registry:
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This sequence was commercially synthesized. Component sequences can be found in the Registry: Yeast FUS1 promoter (BBa_K1154001); Yeast TEF2 promoter (BBa_K165037); Yeast ADH1 terminator (BBa_J63002); Yeast imidazoleglycerol-phosphate dehydratase (IGPD) coding sequence (BBa_K1154002); Human lactate dehydrogenase A (LDHA) coding sequence (BBa_K1154003). However, assembling these sequences using the Composite Parts Tool will not produce a sequence identical to the one submitted here.
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Yeast FUS1 promoter (BBa_K1154001)
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−
Yeast TEF2 promoter (BBa_K165037)
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−
Yeast ADH1 terminator (BBa_J63002)
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−
Yeast imidazoleglycerol-phosphate dehydratase (IGPD)coding sequence (BBa_K1154002)
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−
Human lactate dehydrogenase A (LDHA) coding sequence (BBa_K1154003)
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−
 
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However, assembling these sequences using the Composite Parts Tool will not produce a sequence identical to the one submitted here.
+
  
 
===References===
 
===References===

Latest revision as of 12:00, 20 September 2013

Mating pheromone-induced IGPD and constitutive LDH expression in Yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 763
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was designed for high codon bias in Saccharomyces cerevisiae and conformity with current Registry standards. Its component sequences can be found in the Registry (see Source).

Source

This sequence was commercially synthesized. Component sequences can be found in the Registry: Yeast FUS1 promoter (BBa_K1154001); Yeast TEF2 promoter (BBa_K165037); Yeast ADH1 terminator (BBa_J63002); Yeast imidazoleglycerol-phosphate dehydratase (IGPD) coding sequence (BBa_K1154002); Human lactate dehydrogenase A (LDHA) coding sequence (BBa_K1154003). However, assembling these sequences using the Composite Parts Tool will not produce a sequence identical to the one submitted here.

References