Difference between revisions of "Part:BBa K1132009:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1132009=== | ===Applications of BBa_K1132009=== | ||
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| + | This part that was request to the Registry by Valencia UPV 2015 team. PhiC31 encodes a serine-type recombinase that mediates the sequence-specific recombination between two different attachment sites, called attB and attP. Our team did characterize the functionality of this part in ''N. benthamiana'' plants. To do so, we used PhiC31 as excisionase of our PhiC31-Reporter element ([https://parts.igem.org/Part:BBa_K1742008 BBa_K1742008]) coupled with a GFP tag. After transiently ''Agrobacterium''-mediated transformation in plants, we could observe that a huge percentage of plant cells expressed GFP. See our PhiC31 recombinase module results. | ||
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| + | https://static.igem.org/mediawiki/2015/thumb/4/43/BBa_1742004_GFP_PhiC31.png/800px-BBa_1742004_GFP_PhiC31.png | ||
| + | <small><p><b>Figure 1. Expression levels of GFP in ''N. benthamiana'' leaves. A) Agrobacterium-mediated transformation with the multigenic construct ([https://parts.igem.org/Part:BBa_K1742013 BBa_K1742013]). B) Plant leaf transformed with the PhiC31 reporter element assembled with the promoter, GFP and the terminator.</b></p></small> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 02:50, 18 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1132009
This part that was request to the Registry by Valencia UPV 2015 team. PhiC31 encodes a serine-type recombinase that mediates the sequence-specific recombination between two different attachment sites, called attB and attP. Our team did characterize the functionality of this part in N. benthamiana plants. To do so, we used PhiC31 as excisionase of our PhiC31-Reporter element (BBa_K1742008) coupled with a GFP tag. After transiently Agrobacterium-mediated transformation in plants, we could observe that a huge percentage of plant cells expressed GFP. See our PhiC31 recombinase module results.
Figure 1. Expression levels of GFP in N. benthamiana leaves. A) Agrobacterium-mediated transformation with the multigenic construct (BBa_K1742013). B) Plant leaf transformed with the PhiC31 reporter element assembled with the promoter, GFP and the terminator.
User Reviews
UNIQ76ad8318b4733196-partinfo-00000000-QINU UNIQ76ad8318b4733196-partinfo-00000001-QINU