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| − | __NOTOC__
| + | #REDIRECT [[Help:An Introduction to BioBricks]] |
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| − | [[image:Partinplasmid.png]]
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| − | |width=70%|
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| − | BioBricks are a set of interchangable components, designed with a view to building biological systems in living cells. The picture to the left shows BioBrick BBa_0034 within a plasmid. The yellow arrow represents the antibiotic resistance gene and the green arrow, the plasmid's origin of replication.
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| − | A BioBrick represents a unique sequence of DNA; it might be a gene that codes a protein or a switch triggered by an external input, it also has a unique [[Part Names|name]]. Whatever its [[Part Types|function]], a biobrick comes packaged in a [[Help:Plasmids|plasmid]], ready to be assembled into useful devices & systems.
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| − | ==Building BioBrick Systems==
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| − | Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on [[Assembly:Restriction enzymes|restriction enzymes]], purification, ligation, and transformation - with a twist: BioBrick parts are [[Assembly:Composable|composable]]. The result of assembling two parts is a new part that may be used in future assemblies.
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| − | ;Standard Assembly
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| − | :The classical method of BioBrick assembly, done at the lab bench.
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| − | :Insert pic of std assembly
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| − | ;Automated Assembly
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| − | :Automated assembly, currently in late stage development at iGEM labs.
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| − | :Insert pic of auto assembly
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| − | ==How do I make a BioBrick?==
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| − | ====At the bench====
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| − | Pic of biobrick flanked by coding seq
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| − | Methods:
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| − | *Synthesis
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| − | *PCR
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| − | More information on putting sequence into standardized Biobrick form can be found [[Help:Standardization|here]]
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| − | ====Adding the part to the registry====
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| − | This protocol is quick and easy. A comprehensive guide can be found [[Basic Biobrick, example 1|here]]
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