Difference between revisions of "Part:BBa K1172302:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1172302 short</partinfo>
 
<partinfo>BBa_K1172302 short</partinfo>
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===Design Notes===
 
===Design Notes===
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we isolated the ''ribC'' gene from the genomic sequence of ''shewanella oneidensis'' MR-1. We designed our primers in a way that enabled us to use Gibson Assembly to ligate ''ribC'' with pSB1C3. Insofar, we designed four primers to generate homologous overlaps between pSB1C3 and the ''ribC'' gene:
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::*pSB1C3_fwd_RibC <br> GTTTTAACGGACTATCTTAATACTAGTAGCGGCCGCTGCAGTCC
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::*pSB1C3_rev_RibC <br> TGAACTATACCAGTAAACATCTCTAGAAGCGGCCGCGAATTCC
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::*RibC_fwd <br> GAATTCGCGGCCGCTTCTAGATGTTTACTGGTATAGTTCAAGC
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::*RibC_rev <br> CTGCAGCGGCCGCTACTAGTATTAAGATAGTCCGTTAAAACG
  
  
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===Source===
 
===Source===
  
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shewanella oneidensis MR-1
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''Shewanella oneidensis'' MR-1 genomic sequence.
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*The strain was kindly provided by Dr. Johannes Gescher (Karlsruher Institut für Technologie)
  
 
===References===
 
===References===
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Schicklberger M, Sturm G, Gescher J: '''Genomic Plasticity Enables a Secondary Electron Transport Pathway in ''Shewanella oneidensis'''''. Appl Environ Microbiol., 2013 Feb; vol. 79 no. 4 1150-1159.

Latest revision as of 22:28, 27 October 2013

ribC: 6,7-dimethyl-8-ribityllumazine synthase alpha subunit RibC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 196


Design Notes

we isolated the ribC gene from the genomic sequence of shewanella oneidensis MR-1. We designed our primers in a way that enabled us to use Gibson Assembly to ligate ribC with pSB1C3. Insofar, we designed four primers to generate homologous overlaps between pSB1C3 and the ribC gene:

  • pSB1C3_fwd_RibC
    GTTTTAACGGACTATCTTAATACTAGTAGCGGCCGCTGCAGTCC
  • pSB1C3_rev_RibC
    TGAACTATACCAGTAAACATCTCTAGAAGCGGCCGCGAATTCC
  • RibC_fwd
    GAATTCGCGGCCGCTTCTAGATGTTTACTGGTATAGTTCAAGC
  • RibC_rev
    CTGCAGCGGCCGCTACTAGTATTAAGATAGTCCGTTAAAACG


Source

Shewanella oneidensis MR-1 genomic sequence.

  • The strain was kindly provided by Dr. Johannes Gescher (Karlsruher Institut für Technologie)

References

Schicklberger M, Sturm G, Gescher J: Genomic Plasticity Enables a Secondary Electron Transport Pathway in Shewanella oneidensis. Appl Environ Microbiol., 2013 Feb; vol. 79 no. 4 1150-1159.