Difference between revisions of "Part:BBa K1140003"
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A construction that has a gene coding for the LacI transcription factor, which also has a LVA degradation tag. The LacI gene is transcribed through a constitutive promoter and a thermolabile RBS with an optimal translation temperature of 37°C. Two termination sites were added at the 3’ end of the gene. | A construction that has a gene coding for the LacI transcription factor, which also has a LVA degradation tag. The LacI gene is transcribed through a constitutive promoter and a thermolabile RBS with an optimal translation temperature of 37°C. Two termination sites were added at the 3’ end of the gene. | ||
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| + | ===Usage and Biology=== | ||
| + | RNA thermometers (RNAT) are temperature sensing RNA sequences that regulate translation by preventing the ribosome from binding the transcript until higher temperatures shift it to an open structure. Several naturally occurring RNA thermometers have been described and synthetic sequences that emulate them have been designed and proved to regulate genetic expression at different temperature ranges. The RNAT herein implemented, designed and dubbed u6 by Neupert ''et al.'' [2], is a synthetic sequence that allows translation only at temperatures over 37 °C. This part can be incorporated into a genetic circuit to integrate transcriptional and post-transcriptional regulation, through Tet Repressor and u6-RNAT, respectively. | ||
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Latest revision as of 03:45, 26 September 2013
pCons + 37 oC RNA thermometer + LacI (LVA)
A construction that has a gene coding for the LacI transcription factor, which also has a LVA degradation tag. The LacI gene is transcribed through a constitutive promoter and a thermolabile RBS with an optimal translation temperature of 37°C. Two termination sites were added at the 3’ end of the gene.
Usage and Biology
RNA thermometers (RNAT) are temperature sensing RNA sequences that regulate translation by preventing the ribosome from binding the transcript until higher temperatures shift it to an open structure. Several naturally occurring RNA thermometers have been described and synthetic sequences that emulate them have been designed and proved to regulate genetic expression at different temperature ranges. The RNAT herein implemented, designed and dubbed u6 by Neupert et al. [2], is a synthetic sequence that allows translation only at temperatures over 37 °C. This part can be incorporated into a genetic circuit to integrate transcriptional and post-transcriptional regulation, through Tet Repressor and u6-RNAT, respectively.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 44
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]