Difference between revisions of "Part:BBa K1055000:Experience"

Latest revision as of 18:20, 12 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1055001

When tried to fuse to the CheB signaling protein of E. coli C-terminally, we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG = - 232 kj/mol). Please take a look below:

Hypothetical DNA secondary structure of CheB-mKate fusion protein which might be the reason for continously failing clonation attempts. We derived dG = - 232 kj/mol

User Reviews

UNIQ9c3a8a1a10342ad1-partinfo-00000000-QINU UNIQ9c3a8a1a10342ad1-partinfo-00000001-QINU

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 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 how you used this part and how it worked out.
-===Applications of BBa_K1055000===
+===Applications of BBa_K1055001===
+When tried to fuse to the CheB signaling protein of ''E. coli''  C-terminally, we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG =  - 232 kj/mol). Please take a look below:
+[[Image:CMK_scstr.png|center|400px|thumb|Hypothetical DNA secondary structure of CheB-mKate fusion protein which might be the reason for continously failing clonation attempts. We derived dG =  - 232 kj/mol]]
 ===User Reviews===