Difference between revisions of "Part:BBa K1154002"

Latest revision as of 04:45, 21 September 2013

Saccharomyces cerevisiae imidazoleglycerol-phosphate dehydratase (IGPD)

The Saccharomyces cerevisiae HIS3 gene encodes imidazoleglycerol-phosphate dehydratase (IGPD), which catalyzes the interconversion of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate and 3-(imidazol-4-yl)-2-oxopropyl phosphate plus H2O. The dehydration reaction is a key step in the biosynthesis of histidine in many bacteria, fungi and plants. The gene is routinely used as a selectable marker in yeast histidine auxotrophs harboring a genomic his3 mutation (e.g., his3-Δ1). It also compliments Escherichia coli hisB mutations (e.g., ΔhisB720::kan).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 562
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1154002 short</partinfo>
-The Saccharomyces cerevisiae HIS3 gene encodes imidazoleglycerol-phosphate dehydratase (IGPD), which catalyzes the interconversion of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate and 3-(imidazol-4-yl)-2-oxopropyl phosphate plus H2O. The dehydration reaction is a key step in the biosynthesis of histidine in many bacteria, fungi and plants. The gene is routinely used as a selectable marker in yeast histidine auxotrophs harboring a genomic his3 mutation (e.g., his3-&#916;1). It also compliments Escherichia coli hisB mutations (e.g., &#916;hisB720::kan).
+The ''Saccharomyces cerevisiae HIS3'' gene encodes imidazoleglycerol-phosphate dehydratase (IGPD), which catalyzes the interconversion of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate and 3-(imidazol-4-yl)-2-oxopropyl phosphate plus H2O. The dehydration reaction is a key step in the biosynthesis of histidine in many bacteria, fungi and plants. The gene is routinely used as a selectable marker in yeast histidine auxotrophs harboring a genomic ''his3'' mutation (''e.g.'', ''his3-&#916;1''). It also compliments ''Escherichia coli hisB'' mutations (''e.g.'', ''&#916;hisB720::kan'').
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