Difference between revisions of "Part:BBa K1039017"
 
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HPdO with deleted gVIII Helper Plasmid contains the M13 viral genome with gVIII deleted and it is also lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011). The site from which gVIII was deleted contains a BglII restriction enzyme site.
 
HPdO with deleted gVIII Helper Plasmid contains the M13 viral genome with gVIII deleted and it is also lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011). The site from which gVIII was deleted contains a BglII restriction enzyme site.
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=='''Sequence and Features'''==
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==Sequence and Features==
 
<partinfo>BBa_K1039017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1039017 SequenceAndFeatures</partinfo>
  
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=='''References'''==
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==References==
  
 
#Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA: Inhibition of bacterial conjugation by phage M13 and its Protein g3p: quantitative analysis and model. PLoS One 2011, 6: e19991.
 
#Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA: Inhibition of bacterial conjugation by phage M13 and its Protein g3p: quantitative analysis and model. PLoS One 2011, 6: e19991.

Latest revision as of 22:00, 17 September 2013

HPdO with deleted gVIII Helper Plasmid

HPdO with deleted gVIII Helper Plasmid contains the M13 viral genome with gVIII deleted and it is also lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011). The site from which gVIII was deleted contains a BglII restriction enzyme site.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 7984
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5732
    Illegal PstI site found at 7984
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1707
    Illegal BamHI site found at 2438
    Illegal XhoI site found at 7826
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 7984
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 7984
    Illegal AgeI site found at 5772
    Illegal AgeI site found at 6096
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7989
    Illegal BsaI.rc site found at 6801



References

  1. Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA: Inhibition of bacterial conjugation by phage M13 and its Protein g3p: quantitative analysis and model. PLoS One 2011, 6: e19991.