Difference between revisions of "Part:BBa K1150046"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1150046 short</partinfo>
 
<partinfo>BBa_K1150046 short</partinfo>
  
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|uniCAS RNaimer multiple targets to Bla target 2, <br> Bla target 3, Bla target 4 and VEGF target 4
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|-
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|'''Function'''
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|fourfold targeting with one plasmid
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
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|}
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==Usage and Biology==
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Fusion of [https://parts.igem.org/Part:BBa_K1150045 RNAimer with bla-target 2 and VEGF-target 4] and [https://parts.igem.org/Part:BBa_K1150044 RNAimer with bla-target 3 and bla-target 4]. This RNAimer was build via iGEM-ligation method and contains the DNA-target sequences of three areas of the bla-gene and one area of the VEGF locus. This enables multiple gene targeting throught one plasmid. The transfection with such a multiple target-plasmid avoids additional cell stress compared to transfection with serveral single target-plasmids.
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===Usage and Biology===
 
  
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<span class='h3bb'>
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<span class='h3bb'>Sequence and Features</span>
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==Sequence and Features==
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<partinfo>BBa_K1150046 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1150046 SequenceAndFeatures</partinfo>
  

Latest revision as of 02:34, 5 October 2013

uniCAS RNaimer multiple targets to Bla target 2, 3, 4 and VEGF 4

uniCAS RNaimer multiple targets to Bla target 2,
Bla target 3, Bla target 4 and VEGF target 4
Function fourfold targeting with one plasmid
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]


Usage and Biology

Fusion of RNAimer with bla-target 2 and VEGF-target 4 and RNAimer with bla-target 3 and bla-target 4. This RNAimer was build via iGEM-ligation method and contains the DNA-target sequences of three areas of the bla-gene and one area of the VEGF locus. This enables multiple gene targeting throught one plasmid. The transfection with such a multiple target-plasmid avoids additional cell stress compared to transfection with serveral single target-plasmids.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
    Illegal BglII site found at 1061
    Illegal BglII site found at 1898
    Illegal BglII site found at 2735
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216
    Illegal BsaI.rc site found at 1053
    Illegal BsaI.rc site found at 1890
    Illegal BsaI.rc site found at 2727