Difference between revisions of "Part:BBa J32001:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J32001 short</partinfo>
 
<partinfo>BBa_J32001 short</partinfo>
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===Design Notes===
 
===Design Notes===
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It is a fusion protein so biobrick prefixes and suffixes have been modified to 10 and 24 bp, removing the bp between the flanking restriction sites and the sequence.  Otherwise it would read out of frame with 8bp overlap
 
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===Source===
 
===Source===
  
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The signal sequence PelB and S*Tag sequence came from Novagen.  The antibody sequence is for cAb-CEA5 nanobody from Ablynx.  The C-terminus of Neisseria gonorrhoeae IgA Protease NCBI accession number AE004969
 
  
 
===References===
 
===References===

Latest revision as of 04:57, 12 July 2006

Targeted Localization


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2471
    Illegal PstI site found at 2597
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1391
    Illegal PstI site found at 2471
    Illegal PstI site found at 2597
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1669
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2471
    Illegal PstI site found at 2597
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2471
    Illegal PstI site found at 2597
    Illegal NgoMIV site found at 1079
    Illegal NgoMIV site found at 1680
    Illegal NgoMIV site found at 2305
    Illegal AgeI site found at 2080
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1146
    Illegal BsaI.rc site found at 724
    Illegal BsaI.rc site found at 1357
    Illegal SapI.rc site found at 1561


Design Notes

Source

References