Difference between revisions of "Part:BBa J45993:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J45993 short</partinfo>
 
<partinfo>BBa_J45993 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'
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*Constructed by primer annealing and extension
  
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Total Length: 49 bp
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====Template====
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None.
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Annealing Length: 21 bp
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GC Content: 38.1%
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Melting Temperature: 49.5 degrees C
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hairpin deltaG: -2.66 kcal/mol
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self dimer deltaG: -99.97 kcal/mol
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Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'
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Total Length: 54 bp
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Annealing Length: 25 bp
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GC Content: 20.0%
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Melting Temperature: 46.4 degrees C
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hairpin deltaG: -2.86 kcal/mol
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self dimer deltaG: -99.44 kcal/mol
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heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
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 +
====Forward Primer====
 +
<code>5'- CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA -3'</code>
  
 +
====Reverse Primer====
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<code>5'- GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T -3'</code>
  
 
===Source===
 
===Source===
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*Promoter controlling expression of ''osmY'' from ''Escherichia coli'' (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278])
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This part was PCRed out of E. coli's genome with the primers listed in the Design Notes section.
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===References===
 
===References===
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<biblio>
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#Yim-1994 pmid=8282684
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#Schellhorn-1998 pmid=9829938
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#Wise pmid=8631665
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#Funabashi pmid=11879714
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#Makinoshima pmid=11985708
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#Yim-1992 pmid=1317380
 +
#Hengge-Aronis-1993 pmid=8416901
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#Vijaykumar-2004 pmid=15576800
 +
</biblio>
  
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=453110&dopt=GenBank
 
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http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8282684
 
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http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829938
 
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http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.2002.02746.x
 
  
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http://www.pubmedcentral.org/articlerender.fcgi?artid=178012
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]
  
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http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3C-456T3CY-3&_coverDate=04%2F25%2F2002&_alid=423325879&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=4943&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=7d6f4bac2242f1baa6b2b15260904531
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Neidhart, Frederick C. ''Escherichia coli and Salmonella''. Washington: ASM Press, 1996.

Latest revision as of 23:38, 7 March 2008

Minimal stationary phase osmY promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Constructed by primer annealing and extension

Template

None.

Forward Primer

5'- CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA -3'

Reverse Primer

5'- GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T -3'

Source

  • Promoter controlling expression of osmY from Escherichia coli (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278])

References

<biblio>

  1. Yim-1994 pmid=8282684
  2. Schellhorn-1998 pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708
  6. Yim-1992 pmid=1317380
  7. Hengge-Aronis-1993 pmid=8416901
  8. Vijaykumar-2004 pmid=15576800

</biblio>


[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.