Difference between revisions of "Part:BBa K1017781"
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<partinfo>BBa_K1017781 short</partinfo> | <partinfo>BBa_K1017781 short</partinfo> | ||
| − | LacI is used to regulate Plac. | + | Plac is a promoter regulated by LacI and CAP protein, it has two binding site, one for CAP protein(general present in E.coli) and the other for LacI CAP protein. In the absence of LacI, this part will promote transcription. In the presence of LacI, this part will prohibit transcription. LacI is used to regulate Plac, and it will be inhibited by IPTG(Isopropyl-beta-D-thiogalactopyranoside). J61048 is a terminator. |
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Latest revision as of 08:23, 23 September 2013
LacI+J61048+Plac
Plac is a promoter regulated by LacI and CAP protein, it has two binding site, one for CAP protein(general present in E.coli) and the other for LacI CAP protein. In the absence of LacI, this part will promote transcription. In the presence of LacI, this part will prohibit transcription. LacI is used to regulate Plac, and it will be inhibited by IPTG(Isopropyl-beta-D-thiogalactopyranoside). J61048 is a terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1147
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1161
- 1000COMPATIBLE WITH RFC[1000]