Difference between revisions of "Part:BBa K1060003:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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===Applications of BBa_K1060003===
 
  
 
===User Reviews===
 
===User Reviews===
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<partinfo>BBa_J45700 AddReview 5</partinfo>
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<I>[[User:SanderW|Sander Wuyts]]</I>
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The KU Leuven iGEM 2013 team tried to perform a qPCR on this part. It was impossible to remove the original plasmid DNA, after RNA isolation, even after several attempts with different DNase treatments. This problem is probably due to the fact that this part was provided in a high copy number backbone. If you want to perform a qPCR yourself, we recommend you to clone this part in another backbone or in the genome itself.
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Latest revision as of 00:20, 29 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQ39d0e3c8ff128c30-partinfo-00000000-QINU UNIQ39d0e3c8ff128c30-partinfo-00000001-QINU

•••••

Sander Wuyts

The KU Leuven iGEM 2013 team tried to perform a qPCR on this part. It was impossible to remove the original plasmid DNA, after RNA isolation, even after several attempts with different DNase treatments. This problem is probably due to the fact that this part was provided in a high copy number backbone. If you want to perform a qPCR yourself, we recommend you to clone this part in another backbone or in the genome itself.

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