Difference between revisions of "Part:BBa K1073019"

 
 
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<partinfo>BBa_K1073019 short</partinfo>
 
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To the synthetase for the autoinducer N-butyryl-HSL, which binds to RhlR, (Part:BBa_C0071) a RBS and a double terminator was added to facilitate the construction of an expression system.
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The RhlI protein is a synthetase which produces the chemical autoinducer signal N-butyryl-HSL. In this device it is combined with a RBS and a double terminator in order to facilitate the construction of an expression cassette containing RhlI.  
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===Usage and Biology===
 
===Usage and Biology===
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In combination with a promoter of your choice this device can be used to create a RhlI expression cassette. The RBS used will tune a medium expression level. Rate of transcription can be tuned by strength of promoter. The double terminator will prevent further transcription downstream of RhlI.
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The N-butyryl-HSL autoinducer molecules produced by RhlI bind to RhlR. This complex will bind to specific sequences in the regulatory region of the rhl promoter and induce gene expression.
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Latest revision as of 09:02, 17 October 2013

Autoinducer synthetase RhlI ( producing N-butyryl-HSL) with RBS and double terminator

The RhlI protein is a synthetase which produces the chemical autoinducer signal N-butyryl-HSL. In this device it is combined with a RBS and a double terminator in order to facilitate the construction of an expression cassette containing RhlI.


Usage and Biology

In combination with a promoter of your choice this device can be used to create a RhlI expression cassette. The RBS used will tune a medium expression level. Rate of transcription can be tuned by strength of promoter. The double terminator will prevent further transcription downstream of RhlI.

The N-butyryl-HSL autoinducer molecules produced by RhlI bind to RhlR. This complex will bind to specific sequences in the regulatory region of the rhl promoter and induce gene expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 680
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]