Difference between revisions of "Part:BBa K1189000:Design"
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<partinfo>BBa_K1189000 short</partinfo> | <partinfo>BBa_K1189000 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | On the iGEM parts registry we found TALA (BBa_K782004) that the Slovenian team synthesized for a previous project. TALEs are very large proteins and take a long time to design and synthesize. Therefore, we decided to use these TALEs to test our system. We also saw this as an opportunity to use and build upon parts made by former iGEM teams. | |
===Source=== | ===Source=== | ||
| − | + | TALA (BBa_K782004) was from parts registry. | |
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===References=== | ===References=== | ||
| + | <li>Bogdanove, A. J., Schornack, S., & Lahaye, T. (2010). TAL effectors: finding plant genes for disease and defense. <i>Current Opinion in Plant Biology, 13</i>(4), 394–401. doi:10.1016/j.pbi.2010.04.010 | ||
| + | <li>Mussolino, C., & Cathomen, T. (2012). TALE nucleases: tailored genome engineering made easy. <i>Current Opinion in Biotechnology, 23</i>(5), 644–50. doi:10.1016/j.copbio.2012.01.013 | ||
Latest revision as of 01:31, 26 October 2013
TALE-A with a his tag under a lacI promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2654
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
On the iGEM parts registry we found TALA (BBa_K782004) that the Slovenian team synthesized for a previous project. TALEs are very large proteins and take a long time to design and synthesize. Therefore, we decided to use these TALEs to test our system. We also saw this as an opportunity to use and build upon parts made by former iGEM teams.
Source
TALA (BBa_K782004) was from parts registry.