Difference between revisions of "An Introduction to BioBricks"

(Building BioBrick Systems)
 
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==What is a BioBrick?==
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#REDIRECT [[Help:An Introduction to BioBricks]]
The BioBrick logic family is a set of interchangable components, designed with a view to building biological systems in living cells. The members of this family are designed to be compatible, composible, interchangeable and independent; in this way a new generation of biological engineers are building novel devices much in the  way their electronically-orientated collegues might piece together transistors, resistors and capacitors.
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A BioBrick represents a unique sequence of DNA; it might be a gene that codes a protein or a switch triggered by an external input, it also has a unique [[Part Names|name]]. Whatever its [[Part Types|function]], a biobrick comes packaged in a [[Help:Plasmids|plasmid]], ready to be assembled into useful devices & systems.
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==Building BioBrick Systems==
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Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on [[Assembly:Restriction enzymes|restriction enzymes]], purification, ligation, and transformation - with a twist: BioBrick parts are [[Assembly:Composable|composable]].  The result of assembling two parts is a new part that may be used in future assemblies.
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<b>Standard Assembly</b><i>The classical method of BioBrick assembly, done at the lab bench</i>
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Insert pic of std assembly
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<b>Automated Assembly</b><i>Automated assembly, currently in late stage development at iGEM labs</i>
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Insert pic of auto assembly
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==How do I make a BioBrick?==
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===At the bench===
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Pic of biobrick flanked by coding seq
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Methods:
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*Synthesis
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*PCR
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===Adding the part to the registry===
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This protocol is quick and easy. A comprehensive guide can be found [[Basic Biobrick, example 1|here]]
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Latest revision as of 20:22, 28 November 2007