|
|
| (84 intermediate revisions by 5 users not shown) |
| Line 1: |
Line 1: |
| − | ==What is a BioBrick?==
| + | #REDIRECT [[Help:An Introduction to BioBricks]] |
| − | The BioBrick logic family is a set of interchangable components, designed with a view to building biological systems in living cells. The members of this family are designed to be compatible, composible, interchangeable and independent; in this way a new generation of biological engineers are building novel devices much in the way their electronically-orientated collegues might piece together transistors, resistors and capacitors.
| + | |
| − | | + | |
| − | A BioBrick represents a unique sequence of DNA; it might be a gene that codes a protein or a switch triggered by an external input, it also has a unique [[Part Names|name]]. Whatever its [[Part Types|function]], a biobrick comes packaged in a [[Help:Plasmids|plasmid]], ready to be assembled into useful devices & systems.
| + | |
| − | | + | |
| − | ==Building BioBrick Systems==
| + | |
| − | Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on [[Assembly:Restriction enzymes|restriction enzymes]], purification, ligation, and transformation - with a twist: BioBrick parts are [[Assembly:Composable|composable]]. The result of assembling two parts is a new part that may be used in future assemblies.
| + | |
| − | | + | |
| − | <b>Standard Assembly</b><i>The classical method of BioBrick assembly, done at the lab bench</i>
| + | |
| − | | + | |
| − | Insert pic of std assembly
| + | |
| − | | + | |
| − | ==How do I make a BioBrick?==
| + | |
| − | ===At the bench===
| + | |
| − | | + | |
| − | Pic of biobrick flanked by coding seq
| + | |
| − | | + | |
| − | Methods:
| + | |
| − | *Synthesis
| + | |
| − | *PCR
| + | |
| − | | + | |
| − | ===Adding the part to the registry===
| + | |
| − | This protocol is quick and easy. A comprehensive guide can be found [[Basic Biobrick, example 1|here]]
| + | |
