Difference between revisions of "Part:BBa K1030002"

 
 
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<partinfo>BBa_K1030002 short</partinfo>
 
<partinfo>BBa_K1030002 short</partinfo>
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We use a synthetic constitutive promoter J23102 to drive the expression of tetA, and sfGFP. tetA could transport extracellular Nickel ion into cell and nickel is toxic to E coli. An asv degradation tag is added to the c-terminal of tetA. sfGFP is used to monitor tetA expression in real time using fluorescent plate reader, flow cytometer and microscope.
 
We use a synthetic constitutive promoter J23102 to drive the expression of tetA, and sfGFP. tetA could transport extracellular Nickel ion into cell and nickel is toxic to E coli. An asv degradation tag is added to the c-terminal of tetA. sfGFP is used to monitor tetA expression in real time using fluorescent plate reader, flow cytometer and microscope.
  
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<p>See more details in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1030000 BBa_K1030000]. We did them as a group.</p>
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 20:39, 27 September 2013

J23102->tetA-asv+sfGFP

We use a synthetic constitutive promoter J23102 to drive the expression of tetA, and sfGFP. tetA could transport extracellular Nickel ion into cell and nickel is toxic to E coli. An asv degradation tag is added to the c-terminal of tetA. sfGFP is used to monitor tetA expression in real time using fluorescent plate reader, flow cytometer and microscope.

See more details in BBa_K1030000. We did them as a group.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 960
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 406
    Illegal NgoMIV site found at 566
    Illegal NgoMIV site found at 934
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1273