Difference between revisions of "Part:BBa K1116002:Experience"

(→‎Applications of BBa_K1116002)
(→‎Applications of BBa_K1116002)
 
(2 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
−
 
−
===Applications of BBa_K1116002===
 
−
In our project, BBa_K1116002 serves as an auxiliary node. Our design as follows (Circuit A). Besides, BBa_K1116003 is used in our control design (Circuit B).  The difference between BBa_K1116002 and BBa_K1116003 is the T21 targets.
 
−
 
−
[[File:Figure A.jpg]]      [[File:Figure B.jpg]]
 
−
 
−
By testing the mean value of EYFP (Enhance Yellow Fluorescent Protein) and the relationship between the EYFP and the DNA copy number, we can know whether the plasmids work or not.
 
−
In our design, we use mkate (another fluorescent protein), which radiats red lights, as reference gene to reflect copy number. The results as follows. We can distinguish the mean valve of EYFP of circuit A and circuit B from the following figure.In the picture,1,
 
−
2,3 are the results of Circuit A and 4,5,6 represent Circuit B
 
−
 
−
[[File:Figure C.jpg]]
 
−
 
−
We can know the miRNA-21 targets at T21,leading the decrease of gene lacI. So the expression of EYFP enhanced, thus the plasmid K1116002 works. Equally, we can prove the K1116003 is right.
 
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 07:48, 26 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQb82119370643bdff-partinfo-00000000-QINU UNIQb82119370643bdff-partinfo-00000001-QINU