Difference between revisions of "Part:BBa K1195000"
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<partinfo>BBa_K1195000 short</partinfo>
 
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This is an enzyme that is know to degrade biofilms. We are testing it for its ability to degrade Cholera biofilm.
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This part contains the ''Dispersin B'' gene (''DspB'') from Aggregatibacter actinomycetemcomitans with the signal sequence removed for expression inside the cell. 
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The fundamental component of bacterial biofilms is the extracellular polymeric substance (EPS), which is composed primarily of exopolysaccharides and proteins. The EPS forms the framework matrix for bacterial biofilms, and composes 50% to 90% of the total organic matter of the biofilm.
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DspB is a glycoside hydrolase that catalyzes the hydrolysis of the poly-''N''-acetylglucosamine polysaccharide. This polysaccharide is excreted by both Gram-negative and Gram-positive bacteria, and helps compose the extracellular polymeric substance that is the fundamental component of bacterial biofilms.
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K1195000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1195000 SequenceAndFeatures</partinfo>
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atgaattgttgcgtaaaaggcaattccatatatccgcaaaaaacaagtaccaagcagaccggattaatgctggacatcgcccgacatttttattcacccgaggtgattaaatcctttattgataccatcagcctttccggcggtaattttctgcacctgcatttttccgaccatgaaaactatgcgatagaaagccatttacttaatcaacgtgcggaaaatgccgtgcagggcaaagacggtatttatattaatccttataccggaaagccattcttgaattatcggcaacttgacgatatcaaagcctatgctaaggcaaaaggcattgagttgattcccgaacttgacagcccgaatcacatgacggcgatctttaaactggtgcaaaaagacagaggggtcaagtaccttcaaggattaaaatcacgccaggtagatgatgaaattgatattactaatgctgacagtattacttttatgcaatctttaatgagtgaggttattgatatttttggcgacacgagtcagcattttcatattggtggcgatgaatttggttattctgtggaaagtaatcatgagtttattacgtatgccaataaactatcctactttttagagaaaaaagggttgaaaacccgaatgtggaatgacggattaattaaaaatacttttgagcaaatcaacccgaatattgaaattacttattggagctatgatggcgatacgcaggacaaaaatgaagctgccgagcgccgtgatatgcgggtcagtttgccggagttgctggcgaaaggctttactgtcctgaactataattcctattatctttacattgttccgaaagcttcaccaaccttctcgcaagatgccgcctttgccgccaaagatgttataaaaaattgggatcttggtgtttgggatggacgaaacaccaaaaaccgcgtacaaaatactcatgaaatagccggcgcagcattatcgatctggggagaagatgcaaaagcgctgaaagacgaaacaattcagaaaaacacgaaaagtttattggaagcggtgattcataagacgaatggggatgagtga
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Latest revision as of 06:13, 28 September 2013

Dispersin B

This part contains the Dispersin B gene (DspB) from Aggregatibacter actinomycetemcomitans with the signal sequence removed for expression inside the cell.

The fundamental component of bacterial biofilms is the extracellular polymeric substance (EPS), which is composed primarily of exopolysaccharides and proteins. The EPS forms the framework matrix for bacterial biofilms, and composes 50% to 90% of the total organic matter of the biofilm.

DspB is a glycoside hydrolase that catalyzes the hydrolysis of the poly-N-acetylglucosamine polysaccharide. This polysaccharide is excreted by both Gram-negative and Gram-positive bacteria, and helps compose the extracellular polymeric substance that is the fundamental component of bacterial biofilms.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 970
  • 1000
    COMPATIBLE WITH RFC[1000]