Difference between revisions of "Part:BBa K1104207:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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This part: AhpCp1 is one of the improvement versions of BioBrick Part: ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104207 Part:BBa_K1104207]) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae''].
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[[File: NYMU_47.png|frame|center|'''AhpCp1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104247 Part:BBa_K1104247])''']]
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We use the device: AhpCp1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104247 Part:BBa_K1104247]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104207 Part:BBa_K1104207]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCp1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104207 Part:BBa_K1104207]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
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We tested the improved promoter: AhpCp1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.
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==Results==
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This is the relative promoter strength of AhpC1p(BBa_K1104207) under different H<sub>2</sub>0<sub>2</sub> concentrations tested using AhpC1p+E0840 ([https://parts.igem.org/Part:BBa_K1104247 Part:K1104247]) and found that there is a trend where the higher the H<sub>2</sub>0<sub>2</sub> concentration, the higher the promoter expression in a slight amount:
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[[File: cp1.png|frame|center]]
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Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:
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[[File: NYMU_all1.png|frame|center|Comparison between ahpC and its improvements. ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104247 Part:BBa_K1104247])]]
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Promoter testing of ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) and its improvements.
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[[File: NYMU_AA.png|frame|center]][[File: NYMU_AAAA.png|frame|center]]
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==Discussion==
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===Applications of BBa_K1104207===
 
===Applications of BBa_K1104207===

Latest revision as of 21:52, 27 October 2013


This part: AhpCp1 is one of the improvement versions of BioBrick Part: ahpC promoter (Part:K362001) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp1 (Part:BBa_K1104207) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].

AhpCp1 +E0840 (Part:BBa_K1104247)

We use the device: AhpCp1 +E0840 (Part:BBa_K1104247) includes promoter (Part:BBa_K1104207) and Part:BBa_E0840, induce the promoter by different concentrations of H2O2, and identify the strength. After AhpCp1 (Part:BBa_K1104207) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.

We tested the improved promoter: AhpCp1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.

Results

This is the relative promoter strength of AhpC1p(BBa_K1104207) under different H202 concentrations tested using AhpC1p+E0840 (Part:K1104247) and found that there is a trend where the higher the H202 concentration, the higher the promoter expression in a slight amount:

Cp1.png

Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:

Comparison between ahpC and its improvements. (Part:BBa_K1104247)

Promoter testing of ahpC promoter (Part:K362001) and its improvements.

NYMU AA.png
NYMU AAAA.png

Discussion

Applications of BBa_K1104207

User Reviews

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