Difference between revisions of "Part:BBa K1152000"

 
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1152000 short</partinfo>
 
<partinfo>BBa_K1152000 short</partinfo>
  
−
This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of Brevibacillus Parabrevis.  
+
This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of ''Brevibacillus parabrevis''.  
−
It is the first module in this cluster for the NonRibosomalPeptide-Synthetase of the cyclic decamer Tyrocidine.
+
It is the first module in gene cluster encoding the non-ribosomal peptide (NRP) synthetase of the cyclic decamer Tyrocidine.
−
This specific module Tyca_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP.
+
This specific module TycA_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP.
−
It consists of the A- (adenylation), PCP- (peptide carrier protein) and the E- (epimerization) domain. After Tyc A follows the TycB gene with three modules.  
+
It consists of an A- (adenylation), a PCP- (peptide-carrier protein) and an E- (epimerization) domain. After tycA follows the tycB gene with three modules.  
  
−
This bio brick can be used for the design of different NRPS.  
+
This biobrick can be used for the design of different NRP synthetases.  
−
It was used in the synthetic Tripeptide I of our project.
+
It was used in the synthetic Tripeptide I of our [http://2013.igem.org/Team:Heidelberg/Project/Tyrocidine project].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:07, 4 October 2013

TycA_deltaCom Module from Tyrocidine

This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of Brevibacillus parabrevis. It is the first module in gene cluster encoding the non-ribosomal peptide (NRP) synthetase of the cyclic decamer Tyrocidine. This specific module TycA_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP. It consists of an A- (adenylation), a PCP- (peptide-carrier protein) and an E- (epimerization) domain. After tycA follows the tycB gene with three modules.

This biobrick can be used for the design of different NRP synthetases. It was used in the synthetic Tripeptide I of our [http://2013.igem.org/Team:Heidelberg/Project/Tyrocidine project].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2518