Difference between revisions of "Part:BBa K1117009"
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<partinfo>BBa_K1117009 short</partinfo> | <partinfo>BBa_K1117009 short</partinfo> | ||
| − | + | A strong constitutive promoter J23119 linked to the 42℃ RNA thermometer K115001. | |
| + | ---- | ||
| + | We tested the RNA thermometer K115001 which control gene to initiate translation at 42°C, but we found that the SD region is occluded at 30°C, partial melting occurs at 37 °C, whereas an increase to 42 °C facilitates the mRNA–ribosome interaction owing to full liberation of the SD and AUG start codon. | ||
| + | [[File:BIT-China-k1117009.jpg|400px|]] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 14:30, 25 September 2013
J23119-K115001
A strong constitutive promoter J23119 linked to the 42℃ RNA thermometer K115001.
We tested the RNA thermometer K115001 which control gene to initiate translation at 42°C, but we found that the SD region is occluded at 30°C, partial melting occurs at 37 °C, whereas an increase to 42 °C facilitates the mRNA–ribosome interaction owing to full liberation of the SD and AUG start codon.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]