Difference between revisions of "Part:BBa K1119006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | no | + | <span style="color:red">Caution: If this promoter is fused to a mammalian translation unit using RFC10, the 5'UTR would only have 6nt. If users encounter lower or no expression upon assembly, including extra DNA spacer sequences between the CMV promoter and the first ATG codon might help.</span> |
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===Source=== | ===Source=== | ||
| − | + | The CMV promoter sequence was cloned out from pEGFP-N1(Clonetech) using PCR with primers that includes prefix and suffix in RFC10 standard. | |
===References=== | ===References=== | ||
| + | Clonetech.(1999).pEGFP-N1 Vector Information.Retrieved from | ||
| + | http://www.pkclab.org/PKC/vector/pEGFPN1.pdf | ||
Latest revision as of 15:27, 26 September 2013
CMV promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Caution: If this promoter is fused to a mammalian translation unit using RFC10, the 5'UTR would only have 6nt. If users encounter lower or no expression upon assembly, including extra DNA spacer sequences between the CMV promoter and the first ATG codon might help.
Source
The CMV promoter sequence was cloned out from pEGFP-N1(Clonetech) using PCR with primers that includes prefix and suffix in RFC10 standard.
References
Clonetech.(1999).pEGFP-N1 Vector Information.Retrieved from http://www.pkclab.org/PKC/vector/pEGFPN1.pdf