Difference between revisions of "Part:BBa K1149005"
Margarita K (Talk | contribs) |
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<partinfo>BBa_K1149005 short</partinfo> | <partinfo>BBa_K1149005 short</partinfo> | ||
| − | This part can express a plastic degradation enzyme from Comamonas acidovorans that breaks down ester bonds in polyurethane. The sequence was codon optimized for E.coli. We added a pelB tag to ensure secretion to the N terminal and we have added a 6xpolyhistidine tag to the C terminal. | + | This part can express a plastic degradation enzyme from Comamonas acidovorans that breaks down ester bonds in polyurethane. It containes a Gly-X-Ser-X-Gly motif characteristic of serine hydrolases. The sequence was codon optimized for E.coli. We added a pelB tag to ensure secretion to the N terminal and we have added a 6xpolyhistidine tag to the C terminal. |
The promoter is arabinose inducible. | The promoter is arabinose inducible. | ||
Theoretical pathway of PUR degradation by esterase: | Theoretical pathway of PUR degradation by esterase: | ||
| − | https://static.igem.org/mediawiki/ | + | https://static.igem.org/mediawiki/parts/3/31/PUR_esterase_theoretical_pathway_2.jpg |
| − | + | ||
<p>references: </p> | <p>references: </p> | ||
| − | + | ||
<p> [http://www.sciencedirect.com/science/article/pii/S0922338X99890011 Cloning and sequence analysis of a polyurethane esterase of Comamonas acidovorans 1998] </p> | <p> [http://www.sciencedirect.com/science/article/pii/S0922338X99890011 Cloning and sequence analysis of a polyurethane esterase of Comamonas acidovorans 1998] </p> | ||
<p> [http://www.sciencedirect.com/science/article/pii/S0964830598000663 Purification and characterization of a soluble polyurethane degrading enzyme from Comamonas acidovorans 1999] </p> | <p> [http://www.sciencedirect.com/science/article/pii/S0964830598000663 Purification and characterization of a soluble polyurethane degrading enzyme from Comamonas acidovorans 1999] </p> | ||
Latest revision as of 19:10, 15 September 2013
pelB-pudA expression
This part can express a plastic degradation enzyme from Comamonas acidovorans that breaks down ester bonds in polyurethane. It containes a Gly-X-Ser-X-Gly motif characteristic of serine hydrolases. The sequence was codon optimized for E.coli. We added a pelB tag to ensure secretion to the N terminal and we have added a 6xpolyhistidine tag to the C terminal. The promoter is arabinose inducible.
Theoretical pathway of PUR degradation by esterase:
references:
[http://www.sciencedirect.com/science/article/pii/S0922338X99890011 Cloning and sequence analysis of a polyurethane esterase of Comamonas acidovorans 1998]
[http://www.sciencedirect.com/science/article/pii/S0964830598000663 Purification and characterization of a soluble polyurethane degrading enzyme from Comamonas acidovorans 1999]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 529
Illegal AgeI site found at 604
Illegal AgeI site found at 1753 - 1000COMPATIBLE WITH RFC[1000]