Difference between revisions of "Part:BBa K1157014"

 
 
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<partinfo>BBa_K1157014 short</partinfo>
 
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This biosensor is built to sense C-12 AHL which originates from Pseudomonas aeruginosa. It uses GFP as a reporter, and constitutively express Rhl receptor. When binding with AHL molecules, Rhl receptor will regulate promoter sequence(pRhl) and express GFP which can be detected.  
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This biosensor is built to sense C-12 AHL which originates from Pseudomonas aeruginosa. It uses GFP as a reporter, and constitutively express Rhl receptor. When binding with AHL molecules, Rhl receptor will regulate promoter sequence(pRhl) and express GFP which can be detected. A positive-feedback design is involved in order to sense low-level AHL.
  
 
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Latest revision as of 16:04, 22 September 2013

pLas-LasR-GFPmut3-Pc-LasR

This biosensor is built to sense C-12 AHL which originates from Pseudomonas aeruginosa. It uses GFP as a reporter, and constitutively express Rhl receptor. When binding with AHL molecules, Rhl receptor will regulate promoter sequence(pRhl) and express GFP which can be detected. A positive-feedback design is involved in order to sense low-level AHL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 1810
    Illegal NheI site found at 1833
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 508
    Illegal NgoMIV site found at 2189
    Illegal AgeI site found at 705
    Illegal AgeI site found at 2386
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1582