Difference between revisions of "Part:BBa K1117004"
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ThiF acts like an E1 ubiquitin-activating enzyme for ThiS, which is a ubiquitin-like protein in prokaryotes. The process of ubiquitination can target denatured proteins and stimulate their degradation. | ThiF acts like an E1 ubiquitin-activating enzyme for ThiS, which is a ubiquitin-like protein in prokaryotes. The process of ubiquitination can target denatured proteins and stimulate their degradation. | ||
| + | ---- | ||
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| + | We verified the function of ThiF by observing the growth of E.coli at 40℃，43℃ and 46℃. | ||
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| + | [[File:BIT-China-ThiF-2.jpg|400px|]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 14:45, 25 September 2013
ThiF
ThiF acts like an E1 ubiquitin-activating enzyme for ThiS, which is a ubiquitin-like protein in prokaryotes. The process of ubiquitination can target denatured proteins and stimulate their degradation.
We verified the function of ThiF by observing the growth of E.coli at 40℃，43℃ and 46℃.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]