Difference between revisions of "Part:BBa K1217000"
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<partinfo>BBa_K1217000 short</partinfo> | <partinfo>BBa_K1217000 short</partinfo> | ||
Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217000, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria. | Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217000, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Latest revision as of 19:00, 15 September 2013
pT7-ppk1(T.forsythia)
Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217000, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria.
Usage and Biology
Testing
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 364
Illegal BglII site found at 1826
Illegal XhoI site found at 108 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1284
Illegal NgoMIV site found at 1596 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 384