Difference between revisions of "Part:BBa K1072023:Experience"

 
 
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===Applications of BBa_K1072023===
 
===Applications of BBa_K1072023===
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<h1>BBa_K2368005</h1>
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<h2>Introduction</h2>
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<partinfo>BBa_K2368005 short</partinfo>
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<P><i>P<sub>fus</sub></i> is an induced promoter from <i>Saccharomyces cerevisiae</i>. In the endogenous MAPK pathway in <i>S.cerevisiae</i>, when STE2 binding with α pheromone, STE12 will stimulate <i>FUS1</i> promoter, thereby initiating the mating of yeast. </P>
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<P>However, the sequence of <i>P<sub>fus</sub></i> contains <i>Pst1</i> restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P>
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<div id="section">
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[[File:T_BIT-China_2017part_K2368005.jpeg|center|300px|标题 ‎]]
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<p style="text-align: center">Fig.1 The sequencing result of <i>P<sub>fus</sub></i></p>
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<p>This part is an improvement of [https://parts.igem.org/Part:BBa_K1072023 BBa_K1072023] of 2013 SCUT team.</p>
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===Usage and Biology===
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<h2>Sequence and Features</h2>
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<partinfo>BBa_K2368005 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2368005 parameters</partinfo>
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===User Reviews===
 
===User Reviews===

Latest revision as of 10:45, 26 October 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1072023

BBa_K2368005

Introduction

Pfus

Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in S.cerevisiae, when STE2 binding with α pheromone, STE12 will stimulate FUS1 promoter, thereby initiating the mating of yeast.

However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)

标题 ‎

Fig.1 The sequencing result of Pfus

This part is an improvement of BBa_K1072023 of 2013 SCUT team.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



User Reviews

UNIQ005a6673bf58d03e-partinfo-00000002-QINU

UNIQ005a6673bf58d03e-partinfo-00000003-QINU