Difference between revisions of "Part:BBa K1157011"

 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1157011 short</partinfo>
 
<partinfo>BBa_K1157011 short</partinfo>
  
This is a biosensor which is built to sense C-4 AHL molecules. This construct has a positive control, in which hopes to be able to magnify AHL signals through inducing the production of more AHL receptors(RhlR).
+
This is a biosensor with blue fluorescent protein, which is built to sense C-4 AHL molecules. This construct has a positive feedback design, which induces more production of AHL receptors(RhlR). We hope it is able to sense tiny AHL signal.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:56, 22 September 2013

Pc-RhlR-pRhl-RhlR-BFP

This is a biosensor with blue fluorescent protein, which is built to sense C-4 AHL molecules. This construct has a positive feedback design, which induces more production of AHL receptors(RhlR). We hope it is able to sense tiny AHL signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 779
    Illegal BsaI site found at 1735