Difference between revisions of "Part:BBa K1100105:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | To test the stochastic dynamics of the constitutive promoters by means of measuring fluorescence intensity of sfGFP using flow cytometry. | |
===Source=== | ===Source=== | ||
| − | + | J23105 from Kit, Insulator: Csy4 loci via PCR, sfGFP from Ding Yu lab at Fudan University | |
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===References=== | ===References=== | ||
| + | Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358. | ||
Latest revision as of 00:01, 28 September 2013
J23105 with insulator and sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To test the stochastic dynamics of the constitutive promoters by means of measuring fluorescence intensity of sfGFP using flow cytometry.
Source
J23105 from Kit, Insulator: Csy4 loci via PCR, sfGFP from Ding Yu lab at Fudan University
References
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.