Difference between revisions of "Part:BBa K1100013"

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	<partinfo>BBa_K1100013 short</partinfo>		<partinfo>BBa_K1100013 short</partinfo>
−		+	<p></p>The RNAOUTS1 is from a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT.
−	coming soon	+	It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.
		+	<p></p>The cI regulated promoter is a strong promoter based on the pR promoter from bacteriohage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription.
		+	[[File:RNA-IN-RNA-OUT.jpg|800px|thumb|center|Figure1. mechanism of RNA-INOUT regulatory system]]
		+	[[File:OUTS1.jpg|400px|thumb|center|Figure2. predicted structure of RNAOUTS1]]
		+	== References ==
		+	<p></p>Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.
	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here

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Latest revision as of 22:12, 27 September 2013

R1051-OUT S1

The RNAOUTS1 is from a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT.

It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.

The cI regulated promoter is a strong promoter based on the pR promoter from bacteriohage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription.

References

Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.

Sequence and Features