Difference between revisions of "Part:BBa K1100064"

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		+	<p></p>It is amongst the mutant library of RNA-OUT, part of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. Earlier studies have suggested that the 5 base pairs in the 5’ end of RNA-IN and in the loop domain of RNA-OUT determine the initiation of RNA duplex formation. The mutation of these 5 base pairs resulted in a library of orthogonal RNA regulators of translation. The translation repression rate of these regulators and the cross-talk of them are shown as followed:
		+	[[File:Repression rate.jpg|600px|thumb|center|Figure1. Repression rate]]
		+	[[File:S31.jpg|400px|thumb|center|Figure2. RNAIN/OUTS31 binding duplex]]
		+	[[File:OUTS31.jpg|400px|thumb|center|Figure3. predicted structure of RNAOUTS31]]
		+
		+	References:
		+	<p></p>Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.
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Latest revision as of 16:09, 26 September 2013

RNA-OUT S31

It is amongst the mutant library of RNA-OUT, part of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. Earlier studies have suggested that the 5 base pairs in the 5’ end of RNA-IN and in the loop domain of RNA-OUT determine the initiation of RNA duplex formation. The mutation of these 5 base pairs resulted in a library of orthogonal RNA regulators of translation. The translation repression rate of these regulators and the cross-talk of them are shown as followed:

References:

Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.

Sequence and Features