Difference between revisions of "Part:BBa K1100006:Design"

(Source)
(Source)
 
(One intermediate revision by the same user not shown)
Line 10: Line 10:
  
 
===Source===
 
===Source===
 
+
Insulator: Csy4 loci via PCR,
The sfGFP is from Ding Yu's Lab at Fudan University
+
B0034 via PCR,
 +
sfGFP from Ding Yu's Lab at Fudan University
  
 
===References===
 
===References===
 +
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006.

Latest revision as of 10:19, 20 September 2013


optimized sfGFP with Csy4 insulator loci


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A optimized sfGFP with a Csy4 loci, can be used as a powerful reporter. You can get this part from BBa_K1100015 and other parts we have submitted.

Source

Insulator: Csy4 loci via PCR, B0034 via PCR, sfGFP from Ding Yu's Lab at Fudan University

References

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006.