Difference between revisions of "Part:BBa K1100010"

Latest revision as of 22:06, 27 September 2013

Csy4 generator (with terminator)

A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034), the Csy4 gene and the teminator (BBa_B0015). The generator is designed to express the Csy4 for a futher usage for the cleavage to the csy4 insulator. Meanwhile, it has a insulator itself to avoid the disturbance between the patterns.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 448
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 164

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1100010 short</partinfo>
-R0040-csy4 insulator-B0034-Csy4-B0015
+A seious of parts are put together onto the pSB1C3 plasmid including the promter([https://parts.igem.org/Part:BBa_R0040 BBa_R0040]),csy4 insulator(16-nt), a RBS([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]), the Csy4 gene and the teminator ([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]).
+The generator is designed to express the Csy4 for a futher usage for the cleavage to the csy4 insulator.
+Meanwhile, it has a insulator itself to avoid the disturbance between the patterns.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+Expression of the Csy4 gene.
 <!-- -->