Difference between revisions of "Part:BBa K1114501"

Latest revision as of 18:06, 24 September 2013

MoClo Level 1 RFP reporter with AE fusion sites

pJ04B2Rm_AE

This is a MoClo Level 1 reporting device containing the Level 0 parts J23104_AB, BCD2_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

This part contains a bicistronic design (BCD) element as the 5' UTR regulatory part from Mutalik et al., 2013. From the library of BCDs, this devices contains a MoClo version of BCD2 (BioFAB # apFAB682) and we obtained the sequence data for this part from the BioFAB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 694
Illegal AgeI site found at 806
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1114501 short</partinfo>
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+pJ04B2Rm_AE <br><br>
 This is a <a href=""http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> Level 1 reporting device containing the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="https://parts.igem.org/Part:BBa_K1114107">BCD2_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114211">E1010m_CD</a>, and <a href="https://parts.igem.org/Part:BBa_K1114300">B0015_DE</a> within the <a href="https://parts.igem.org/Part:BBa_K1114418">DVL1_AE</a> backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
+<br><br>
+This part contains a bicistronic design (BCD) element as the 5' UTR regulatory part from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a>. From the library of BCDs, this devices contains a MoClo version of BCD2 (BioFAB # apFAB682) and we obtained the sequence data for this part from the <a href="http://biofab.org/data">BioFAB</a>.
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