Difference between revisions of "Part:BBa J100129:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_J100129===
 
===Applications of BBa_J100129===
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Our promoter J100129 was not cloned successfully as indicated by the presence of just green fluorescence. We tried to clone it into [[Part:BBa_J119137]], and it we had, the colonies would not be green. Unfortunately, all of ours were green.  The following is a graph detailing the fluorescence per optical density. This is not a measure of our promoter since we did not clone it into the plasmid. This is part of an undergraduate biology lab assignment. Our positive control is [[Part:BBa_J04450]] with a strong promoter.
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[[File:113SP.png]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 21:56, 26 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J100129

Our promoter J100129 was not cloned successfully as indicated by the presence of just green fluorescence. We tried to clone it into Part:BBa_J119137, and it we had, the colonies would not be green. Unfortunately, all of ours were green. The following is a graph detailing the fluorescence per optical density. This is not a measure of our promoter since we did not clone it into the plasmid. This is part of an undergraduate biology lab assignment. Our positive control is Part:BBa_J04450 with a strong promoter.


113SP.png

User Reviews

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