Difference between revisions of "Part:BBa K1017101:Design"
Huierh04102 (Talk | contribs) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1017101 short</partinfo> | <partinfo>BBa_K1017101 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Since the transcription factor of PompC, OmpR, is phosphorylated in dark and non phosphorylated in red light, we used LacI and Plac downstream PompC to reverse the original function of PompC. By this way, we can express the genes downstream in red light and block them in dark, making this biobrick a red light-induced promoter. | |
| Line 13: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | + | Assemble parts form iGEM distribution | |
===References=== | ===References=== | ||
Latest revision as of 12:25, 17 September 2013
Pred ( PompC + B0032 + LacI + J61048 + Plac)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1282
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1296
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since the transcription factor of PompC, OmpR, is phosphorylated in dark and non phosphorylated in red light, we used LacI and Plac downstream PompC to reverse the original function of PompC. By this way, we can express the genes downstream in red light and block them in dark, making this biobrick a red light-induced promoter.
Source
Assemble parts form iGEM distribution