Difference between revisions of "Part:BBa K1124006"

Latest revision as of 04:05, 28 September 2013

anti-mCherry sRNA

anti-mCherry sRNA

You can make your own artificial sRNA BioBrick part to knock down a target gene.

How to make sRNA BioBrick part

1, Choose a target gene you want to repress.
2, The design principle described on our wiki(currently under construction) gives you PCR primers design. 
3, PCR (template: this BioBrick part (pSB1C3 carrying BBa_K1124005), primers: primers you get in 2.)
4, You get your sRNA BioBrick part. (the sRNA BioBrick parts UT-Tokyo 2013 made; BBa_K1124006, BBa_K1124007 ,BBa_K1124008, BBa_K1124009, BBa_K1124010 )

You can use this sRNA BioBrick part as follows;

1, Add a promoter and terminator.
2, You get sRNA generator of BioBrick standard.
3, Transform E. coli with sRNA generator BioBrick part. You will see knock down of the target gene.

sRNA (small RNA) repress the translation by blocking TIR*1 and by facilitating degradation of mRNA by RNase E [1]. Artificial sRNAs can be used in many ways (e.g. metabolic engineering, construction of genetic circuit,......).

MicC is a sRNA of E. coli, which scaffold is used as a template for artificial sRNA synthsis [2].

• 1: TIR = translation initiation region

References [1]Aiba, H. (2007). Mechanism of RNA silencing by Hfq-binding small RNAs. Current opinion in microbiology, 10(2), 134-139.

[2] Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013). Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature biotechnology.

Sequence and Features