Difference between revisions of "Part:BBa K1061013"
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<partinfo>BBa_K1061013 short</partinfo> | <partinfo>BBa_K1061013 short</partinfo> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1061013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1061013 SequenceAndFeatures</partinfo> | ||
| + | ====Funtional experiment==== | ||
| + | We conducted the functional experiment by co-tranfected the plasmid contains tTA and the plasmid contains P tight into Bosc, one kind of HEK-293 cell lines.Noted that we did not use tTA advance due to time limit(we construct the plasmid contains tTA advance later), and we will try that later,for improvement of expression control. | ||
| + | [[Image:P_tight_functional_test.jpg|thumb|center|800px|functional assay of tTA]] | ||
| + | The after adding dox, the expression of dox is almost fully suppressed. | ||
| + | We also get the quantitive data by western blot, which will be submitted later. | ||
| + | ====quantitive analysis==== | ||
| + | <h2>Measurement Contribution</h2> | ||
| + | *'''Group:''' Tsinghua-A, 2016 | ||
| + | *'''Author:''' Yuxi Ke | ||
| + | *'''Summary:''' We quantitatively measured and calculated TRE promotor's ability to accurately transmit information by channel capacity, a concept in information theory, the unit of which is bit. | ||
| + | |||
| + | *'''Link:'''http://2016.igem.org/Team:Tsinghua-A/Description | ||
| + | |||
| + | *'''Our Improvement''' | ||
| + | **'''Purpose:'''Channel capacity evaluates correlation between input and output signals. For instance, if channel capacity is larger than 1 bit, the part can decern between high and low inputs, and vice versa. By assessing this novel quantity, we through light on how much information can a inducible promotor pass on. The measuring and calcualtion methods can be applied to more promotors in the future. | ||
| + | **'''Results:'''Using EBFP2 as reporter, we measure channel capacity of a simple circuit driven by TRE to be 0.5259, with standard deviation of 0.0058. | ||
| + | [[Image:chcaTRE.PNG|thumb|center|800px|channel capacity of TRE]] | ||
| + | **'''Methods''' | ||
| + | *'''Uploads:''' (links to uploads relevant to your contribution, ex: csv containing your data, sequence files, etc.) '''Note:''' All files pertaining to a contribution must be [[Help:Upload_Files|uploaded to the Registry]]. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K1061013 parameters</partinfo> | <partinfo>BBa_K1061013 parameters</partinfo> | ||
| − | < | + | |
| + | <h2>References:</h2> | ||
| + | <p>[1]http://www.clontech.com/CN/Products/Inducible_Systems/Tetracycline-Inducible_Expression/Gen2?sitex=10022:22372:US | ||
Latest revision as of 22:44, 19 October 2016
P tight promoter
It is a response element containing 7 consecutive TetO elements and a minimal CMV promoter. When binding to tTA or rtTA, the expression of the downstream gene will be highly activated. Besides, it can achieve low constitutive expression(almost undectectable) when not binding to tTA or rtTA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Funtional experiment
We conducted the functional experiment by co-tranfected the plasmid contains tTA and the plasmid contains P tight into Bosc, one kind of HEK-293 cell lines.Noted that we did not use tTA advance due to time limit(we construct the plasmid contains tTA advance later), and we will try that later,for improvement of expression control.
The after adding dox, the expression of dox is almost fully suppressed. We also get the quantitive data by western blot, which will be submitted later.
quantitive analysis
Measurement Contribution
- Group: Tsinghua-A, 2016
- Author: Yuxi Ke
- Summary: We quantitatively measured and calculated TRE promotor's ability to accurately transmit information by channel capacity, a concept in information theory, the unit of which is bit.
- Link:http://2016.igem.org/Team:Tsinghua-A/Description
- Our Improvement
- Purpose:Channel capacity evaluates correlation between input and output signals. For instance, if channel capacity is larger than 1 bit, the part can decern between high and low inputs, and vice versa. By assessing this novel quantity, we through light on how much information can a inducible promotor pass on. The measuring and calcualtion methods can be applied to more promotors in the future.
- Results:Using EBFP2 as reporter, we measure channel capacity of a simple circuit driven by TRE to be 0.5259, with standard deviation of 0.0058.
- Methods
- Uploads: (links to uploads relevant to your contribution, ex: csv containing your data, sequence files, etc.) Note: All files pertaining to a contribution must be uploaded to the Registry.