Difference between revisions of "Part:BBa K1124000:Design"

(Created page with "__NOTOC__ <partinfo>BBa_K1124000 short</partinfo> <partinfo>BBa_K1124000 SequenceAndFeatures</partinfo> ===Design Notes=== LVA degradation tag (sequence: AANDENYALVA) was add...")
 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1124000 short</partinfo>
 
<partinfo>BBa_K1124000 short</partinfo>
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<partinfo>BBa_K1124000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1124000 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
  
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LVA degradation tag (sequence: AANDENYALVA) was added to increase protein degradation.  .     
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LVA degradation tag was added to increase protein degradation.  .     
  
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13 amino acids (sequence: RPAANDENYALVA) and double stop codon were added. The first 2 aa (sequence: RP) is Stu1 restriction site[[1]], which may not be necessary to increase protein decay.
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13 amino acids (sequence: RPAANDENYALVA) and double stop codon were added. The first 2 aa (sequence: RP) is Stu1 restriction site, which may not be necessary to increase protein decay. LVA tag is the last 11 aa(sequence: AANDENYALVA) .[[1]]
  
 
===Source===
 
===Source===
 
PCR (template: BBa_K592010, primers are shown on our wiki (UT-Tokyo 2013)(currently under construction). )
 
PCR (template: BBa_K592010, primers are shown on our wiki (UT-Tokyo 2013)(currently under construction). )
−
 
  
 
===References===
 
===References===
 
[[1]] Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.
 
[[1]] Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.

Latest revision as of 02:57, 12 September 2013

amilGFP (+LVA), yellow reporter protein with degradation tag



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LVA degradation tag was added to increase protein degradation. .

13 amino acids (sequence: RPAANDENYALVA) and double stop codon were added. The first 2 aa (sequence: RP) is Stu1 restriction site, which may not be necessary to increase protein decay. LVA tag is the last 11 aa(sequence: AANDENYALVA) .1

Source

PCR (template: BBa_K592010, primers are shown on our wiki (UT-Tokyo 2013)(currently under construction). )

References

1 Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.