Difference between revisions of "Part:BBa K1031421"

 
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1031421 short</partinfo>
 
<partinfo>BBa_K1031421 short</partinfo>
 +
<html>
 +
<p>For detailed information concerning HcaR and Ph promoter, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/HcaR">2013 Peking iGEM Biosensor HcaR</a></p>
  
−
Ph-32-sfGFP-TT (HcaR)
+
<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
 +
</html>
 +
 
 +
 
 +
== '''Structure''' ==
 +
 
 +
<html>
 +
<img src="https://static.igem.org/mediawiki/igem.org/4/41/Peking2013_HcaRFig1.jpg" style="width:600px; margin-left:170px" />
 +
<p style="text-align:center"><b>Fig 1</b> Structure of <i>Ph</i> promoter regulated by HcaR and chemical pathway.
 +
</html>
 +
 
 +
 
 +
 
 +
== '''Sequence and Features''' ==
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1031421 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1031421 SequenceAndFeatures</partinfo>
 +
 +
 +
 +
== '''Construction and data''' ==
 +
 +
For the biosensor circuit ''Ph''/HcaR, we created a library of RBS (Ribosome Binding Site) including B0031, B0032 and B0034 for tuning the expression level of reporter gene sfGFP.
 +
K1031421 is composed of three elements, ''Ph'' promoter activated by HcaR, RBS <html><a href="https://parts.igem.org/Part:BBa_B0032">B0032</a></html> and reporter gene sfGFP ('''Fig 1a'''). Induction ratio was tested when K1031421 is in collocation with J23106-HcaR and J23114-HcaR, as shown in the dashed boxes ('''Fig 1b'''). From data we concluded biosensor circuit ''Ph''/HcaR adopting ''Pc'' J23114 and RBS B0032 obtained the optimal performance.
 +
 +
<html>
 +
<img src="https://static.igem.org/mediawiki/igem.org/thumb/c/ca/Peking2013_part_K1031421.png/800px-Peking2013_part_K1031421.png",  width=950px;  />
 +
<p style="text-align:center"><b>Fig 1</b> Construction and tunning of reporter circuit <i>Ph</i>-B0032-sfGFP. (<b>a</b>) The orange arrow represents <i>Ph</i> promoter activated by HcaR. The green oval stands for RBS B0032. sfGFP coding sequence is shown with dark blue, while terminator B0015 is in dark red. (<b>b</b>) Test was done following <a href="http://2013.igem.org/Team:Peking/Team/Notebook/Protocols">Test Protocol 1</a>. Horizontal axis represents biosensor circuit adopting different combination of <i>Pc</i> promoter and RBS. Vertical axis stands for induction ratio. The dashed boxes refer to data for reporter circuit <i>Pc</i>-B0032-sfGFP in collocation with biosensor circuits J23106-HcaR and J23114-HcaR respectively.
 +
</html>
 +
 +
 +
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K1031421 parameters</partinfo>
+
<partinfo>BBa_K1031420
−
<!-- -->
+

Latest revision as of 22:29, 27 September 2013

Ph-B0032-sfGFP-Terminator (HcaR)

For detailed information concerning HcaR and Ph promoter, please visit 2013 Peking iGEM Biosensor HcaR


Structure

Fig 1 Structure of Ph promoter regulated by HcaR and chemical pathway.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 112


Construction and data

For the biosensor circuit Ph/HcaR, we created a library of RBS (Ribosome Binding Site) including B0031, B0032 and B0034 for tuning the expression level of reporter gene sfGFP. K1031421 is composed of three elements, Ph promoter activated by HcaR, RBS B0032 and reporter gene sfGFP (Fig 1a). Induction ratio was tested when K1031421 is in collocation with J23106-HcaR and J23114-HcaR, as shown in the dashed boxes (Fig 1b). From data we concluded biosensor circuit Ph/HcaR adopting Pc J23114 and RBS B0032 obtained the optimal performance.

Fig 1 Construction and tunning of reporter circuit Ph-B0032-sfGFP. (a) The orange arrow represents Ph promoter activated by HcaR. The green oval stands for RBS B0032. sfGFP coding sequence is shown with dark blue, while terminator B0015 is in dark red. (b) Test was done following Test Protocol 1. Horizontal axis represents biosensor circuit adopting different combination of Pc promoter and RBS. Vertical axis stands for induction ratio. The dashed boxes refer to data for reporter circuit Pc-B0032-sfGFP in collocation with biosensor circuits J23106-HcaR and J23114-HcaR respectively.