Difference between revisions of "Part:BBa K1031744"

 
 
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<p>For detailed information concerning PaaX, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/HcaR#Mileston3">2013 Peking iGEM Biosensor PaaX</a></p>
  
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== '''Construction''' ==
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In order to fine-tune the performance of PaaX biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031744 is composed of three elements, the constitutive ''Pc'' promoter <html><a href="https://parts.igem.org/Part:BBa_J23114">J23114</a></html>, coding sequence of PaaX and terminator <html><a href="https://parts.igem.org/Part:BBa_B0015">B0015</a></html>. ('''Fig 1''')
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<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit <i>Pa</i>/J23114-PaaX. The orange arrowheads represent promoters. RBS are shown in green ovals. The square in dark red stands for terminator B0015.
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== '''Sequence and Features''' ==
  
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===Usage and Biology===
 
  
 
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Latest revision as of 23:44, 27 September 2013

J23114-PaaX

For detailed information concerning PaaX, please visit 2013 Peking iGEM Biosensor PaaX


Construction

In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031744 is composed of three elements, the constitutive Pc promoter J23114, coding sequence of PaaX and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit Pa/J23114-PaaX. The orange arrowheads represent promoters. RBS are shown in green ovals. The square in dark red stands for terminator B0015.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]