Difference between revisions of "Part:BBa K1123004"

 
 
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<partinfo>BBa_K1123004 short</partinfo>
 
<partinfo>BBa_K1123004 short</partinfo>
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===TU-Eindhoven Sequencing Results===
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This construct has been submitted to the iGEM registry and has been privately sequenced by the iGEM 2013 TU-Eindhoven. The sequencing results were positive indicating that the sequence and part submitted to the registry are correct.
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''Sequencing was performed by BaseClear Netherlands''
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1123004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1123004 SequenceAndFeatures</partinfo>
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===Characterization===
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This part is a composite part which is comprised of a number of other important parts namely [[Part:BBa_K1123000 | BBa_K1123000]] and [[Part:BBa_K1123016 | BBa_K1123016]].
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This part has, in itself not been tested by us here at the TU-Eindhoven during the 2013 iGEM competition. However, as the other, important parts which make up this part have been tested, shown to work, and characterized we would like to refer you to these parts for a complete overview of the characterization of this part.
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We do not think there will be a change in the functionality of any of the separate parts when combining them. A similar composite part [[Part:BBa_K1123005 | BBa_K1123005]] was combined and tested and showed no change in functionality other than a decrease in expression.
  
  

Latest revision as of 23:09, 4 October 2013

1G70 Protein Production Construct

This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the 1G70 Protein sequence. The FNR promotor sequence enables us to bring the 1G70 protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The 1G70 protein is part of a HIV protein which we are expressing with the sole purpose of using it as a CEST contrast agent.

TU-Eindhoven Sequencing Results

This construct has been submitted to the iGEM registry and has been privately sequenced by the iGEM 2013 TU-Eindhoven. The sequencing results were positive indicating that the sequence and part submitted to the registry are correct. Sequencing was performed by BaseClear Netherlands

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
    Illegal XhoI site found at 611
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 269
    Illegal NgoMIV site found at 353
    Illegal NgoMIV site found at 437
    Illegal NgoMIV site found at 521
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

This part is a composite part which is comprised of a number of other important parts namely BBa_K1123000 and BBa_K1123016.

This part has, in itself not been tested by us here at the TU-Eindhoven during the 2013 iGEM competition. However, as the other, important parts which make up this part have been tested, shown to work, and characterized we would like to refer you to these parts for a complete overview of the characterization of this part.

We do not think there will be a change in the functionality of any of the separate parts when combining them. A similar composite part BBa_K1123005 was combined and tested and showed no change in functionality other than a decrease in expression.