Difference between revisions of "Part:BBa K1230004:Experience"

 
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Enter the review inofrmation here.
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We were able to characterise this part, since we found a measurable phenotype (kanamycin resistance).
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We also characterised it using Kanamycin �Minimum Inhibitory Concentration� (MIC) finding a MIC with MarA overexpression: 5 µg/ml and a wild-type MIC: 1 µg/ml
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https://static.igem.org/mediawiki/igem.org/8/8b/U102_Fig2.png
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A test was performed with three different antibiotics and different IPTG concentrations.
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The part seems to make the host sensible to Tetracyclin (maybe because it's on Cm plasmid, and both antibiotics act on similar ways), it seems not to have an effect on Ampicilin and it shows a relevant effect with Kanamycin. Which is further explored in the next figure.
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https://static.igem.org/mediawiki/igem.org/2/21/U101_Fig3.png
 
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Latest revision as of 22:07, 5 October 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

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