Difference between revisions of "Part:BBa K1114107:Design"
(→Source) |
(→Design Notes) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 10: | Line 10: | ||
===Design Notes=== | ===Design Notes=== | ||
<html> | <html> | ||
| − | This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site | + | This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 2 with BC fusion sites. |
| Line 29: | Line 29: | ||
===References=== | ===References=== | ||
| − | <a href= | + | <html> |
| + | |||
| + | <a href= "http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html">Mutalik, et al.</a> | ||
| + | |||
| + | </html> | ||
Latest revision as of 20:12, 7 September 2013
Bicistronic Design RBS 2, MoClo Format with BC fusion sites
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is the MoClo formatted part of a bicistronic design ribosomal binding site 2 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
Source
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.