Difference between revisions of "Part:BBa K1114409"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1114409 short</partinfo> | <partinfo>BBa_K1114409 short</partinfo> | ||
+ | <br> | ||
+ | This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites D on the 5' side and site F on the 3' side of the part. | ||
+ | The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. | ||
+ | |||
+ | Summary of modifications from original part: | ||
− | + | Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 04:52, 25 September 2013
DVL0_DG
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites D on the 5' side and site F on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 245
Illegal XbaI site found at 272
Illegal SpeI site found at 1
Illegal SpeI site found at 529
Illegal PstI site found at 284 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 245
Illegal SpeI site found at 1
Illegal SpeI site found at 529
Illegal PstI site found at 284 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 245
Illegal BamHI site found at 266 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 245
Illegal XbaI site found at 272
Illegal SpeI site found at 1
Illegal SpeI site found at 529
Illegal PstI site found at 284 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 245
Illegal XbaI site found at 272
Illegal SpeI site found at 1
Illegal SpeI site found at 529
Illegal PstI site found at 284 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 8
Illegal BsaI.rc site found at 522