Difference between revisions of "Part:BBa K1033120"
m |
|||
(10 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1033120 short</partinfo> | <partinfo>BBa_K1033120 short</partinfo> | ||
− | Construct needed to produce the protein miraculin, | + | Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". |
+ | |||
+ | This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes. | ||
+ | |||
+ | '''Applications''' | ||
+ | |||
+ | Miraculin can be used as a sweetener. | ||
+ | |||
+ | |||
+ | ==Characterization data from Uppsala iGEM 2013== | ||
+ | We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further. | ||
+ | |||
+ | |||
+ | ===Registry Curation=== | ||
+ | Original sequence changed by iGEM HQ | ||
+ | *'''Change''' - "TAT" added to start of part sequence, ''notified by Team [http://2015.igem.org/Team:UMaryland UMaryland 2015 Team]'' | ||
+ | **'''Reason''' - To reflect sample sequence | ||
+ | *'''Change''' - Part type from "Coding" to "Translational Unit" | ||
+ | **'''Reason''' - RBS in front of coding Region | ||
+ | |||
+ | {{Template:CurationNote|change="TAT" added to start of part sequence|reason=To reflect sample sequence, ''notified by [http://2015.igem.org/Team:UMaryland UMaryland 2015 Team]''}} | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 20:20, 7 July 2015
B0034-ExpTag-Linker-Miraculin-Linker-6xHis
Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum".
This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
Applications
Miraculin can be used as a sweetener.
Characterization data from Uppsala iGEM 2013
We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.
Registry Curation
Original sequence changed by iGEM HQ
- Change - "TAT" added to start of part sequence, notified by Team [http://2015.igem.org/Team:UMaryland UMaryland 2015 Team]
- Reason - To reflect sample sequence
- Change - Part type from "Coding" to "Translational Unit"
- Reason - RBS in front of coding Region
HQ Curation
iGEM HQ Staff has curated this part. Curation may add, remove, or alter information on a part, to better represent its use and character. This part may require further curation.
Change: {{{1}}}
- Reason: {{{2}}}
iGEM users are also encouraged to make their own additions and changes to part pages as contributions. Contact hq (AT) igem (DOT) org with any questions.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]