Difference between revisions of "Part:BBa K1014014"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1014014 short</partinfo> | <partinfo>BBa_K1014014 short</partinfo> | ||
| − | hrpL | + | At the beginning, Ptet promoter is on and HrpS forming rate is max,.So HrpS is adequate at that time. Once treated with protein TetR,concentration of HrpS is dropped to a low level. While there will not be HrpR unless treated with IPTG. Only when both HrpS and HrpR are at a high level, can the promoter hrpL trigger the transcription of following genes. This device function like a digital 'AND' gate. |
| + | <br /> | ||
| + | <table cellpadding="3" border="1px" cellspacing="0" align="center" width="70%"> | ||
| + | <caption><p align="justify"><b>Table 1</b> Relationship between input(TetR and IPTG) and output(RFP) </p></caption> | ||
| + | <tr><td><b>!TetR</b></td><td><b>IPTG</b></td><td><b>RFP</b></td></tr> | ||
| + | <tr><td><b>0</b></td><td><b>0</b></td><td><b>0</b></td></tr> | ||
| + | <tr><td><b>0</b></td><td><b>1</b></td><td><b>0</b></td></tr> | ||
| + | <tr><td><b>1</b></td><td><b>0</b></td><td><b>0</b></td></tr> | ||
| + | <tr><td><b>1</b></td><td><b>1</b></td><td><b>1</b></td></tr> | ||
| + | </table> | ||
| + | <br /> | ||
| + | !TetR=1 means TetR concentration is at a low level, !TetR=0 means TetRconcentration is at a high level. | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 08:19, 26 September 2013
hrpL AND Gate device
At the beginning, Ptet promoter is on and HrpS forming rate is max,.So HrpS is adequate at that time. Once treated with protein TetR,concentration of HrpS is dropped to a low level. While there will not be HrpR unless treated with IPTG. Only when both HrpS and HrpR are at a high level, can the promoter hrpL trigger the transcription of following genes. This device function like a digital 'AND' gate.
| !TetR | IPTG | RFP |
| 0 | 0 | 0 |
| 0 | 1 | 0 |
| 1 | 0 | 0 |
| 1 | 1 | 1 |
!TetR=1 means TetR concentration is at a low level, !TetR=0 means TetRconcentration is at a high level.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 618
Illegal AgeI site found at 205
Illegal AgeI site found at 211
Illegal AgeI site found at 259
Illegal AgeI site found at 825
Illegal AgeI site found at 850
Illegal AgeI site found at 1417
Illegal AgeI site found at 1423 - 1000COMPATIBLE WITH RFC[1000]