Difference between revisions of "Help:PoPS"
 
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''Po''lymerase ''P''er ''S''econd: <br>
 
''Po''lymerase ''P''er ''S''econd: <br>
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Transcription initiation is defined as the number of times that an RNA Polymerase passes from the regulatory region and into the protein coding region of a BioBrick gene. PoPS are measured by introducing a test gene with the same regulatory region but with a CFP protein coding region and measuring the fluorescence.
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Polymerase Per Second (or PoPS)  is defined as the number of times that an RNA polymerase molecule passes a specific point on DNA per unit time (e.g., from the 3' end of a promoter part into the 5' end of a downstream part such as a ribosome binding site). PoPS are measured indirectly by introducing a test gene with the same regulatory region but with a CFP protein coding region and measuring the fluorescence.  For more information please see the "Adventures in Synthetic Biology" [http://www.openwetware.org/wiki/Adventures comic].
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This concept is related to [[Help:RiPS]], the ''Ri''bosomes ''P''er ''S''econd of a system. <br>
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An older term which is used synonymously with PoPs is [[Help:TiPS|TiPS]], the ''T''ranscription ''i''nitiations ''P''er ''S''econd of a system.
  
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This concept is related to [[Help:RiPS]], the ''Ri''bosomes ''P''er ''S''econd of a system.
 
  
  
 
<small>More on [[Part Types:Measurement Systems]]...
 
<small>More on [[Part Types:Measurement Systems]]...

Latest revision as of 15:53, 5 October 2006

Polymerase Per Second:
Polymerase Per Second (or PoPS) is defined as the number of times that an RNA polymerase molecule passes a specific point on DNA per unit time (e.g., from the 3' end of a promoter part into the 5' end of a downstream part such as a ribosome binding site). PoPS are measured indirectly by introducing a test gene with the same regulatory region but with a CFP protein coding region and measuring the fluorescence. For more information please see the "Adventures in Synthetic Biology" [http://www.openwetware.org/wiki/Adventures comic].

This concept is related to Help:RiPS, the Ribosomes Per Second of a system.
An older term which is used synonymously with PoPs is TiPS, the Transcription initiations Per Second of a system.


More on Part Types:Measurement Systems...